Coding

Part:BBa_K2433000:Design

Designed by: Tamar Av-Shalom   Group: iGEM17_British_Columbia   (2017-10-25)


ietA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 737
    Illegal XhoI site found at 659
    Illegal XhoI site found at 989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49
    Illegal BsaI.rc site found at 836
    Illegal SapI.rc site found at 373


Design Notes

We also took the native promoter from this gene by taking about 300 bp upstream of the start codon.


Source

Atu6082 from pTiC58(https://www.ncbi.nlm.nih.gov/nuccore/159141737)

References